Hopefully
you will now appreciate the potential the fungi offer in bioremedial
technologies. This can be improved upon in many ways, obviously
further research is needed in situ and in the lab.
However
it is clear that for bioremedial future research, fungi seem to
have to edge over bacteria.
This
can be attributed to different reasons:
-
Fungal
tolerance: they are able to withstand a wide range of environmental
conditions, including oxygen availability, temperature range
and pH. Their tolerance to highly toxic conditions is
far greater than bacteria, and so are able to survive in high
concentrations of pollutants unlike most bacteria.
-
Their
mode of growth, which is by growing hyphae, extends the surface
area available for transformation. Bacteria being single
celled organisms do not have this process.
-
They
degrade a wider range of xenobiotics than bacteria.
-
Their
nutrient source is inexpensive and abundant - lignocellulose.
-
They
do not have to preconditioned or treated to survive in situ
unlike many bacteria.
-
Their
method of transformation is non-specific allowing the degradation
of a mixture of organopollutants in soil, which is what is
likely to be found in the environment.
However
we should not rule out bacterial bioremediation as there is potential
for systems that use both fungi and bacteria for transformation.
There
are also problems to overcome, such as providing sufficient biomass
of fungi for efficient removal of organopollutants. Also
a way of stopping competition from indigenous populations of bacteria
in the soil would improve the biodegrading ability of the fungi.
Future research could also look into different aspects, including
genetics, isolation of fungal enzymes, and possible discovery
of new fungi with better degradative properties.
Genetic analysis of the processes involved in ligninolytic enzymes
could shed light on why certain enzymes are produced, by which
type of fungi, and at what stage. Also there are genetic engineering
possibilities, such as transferring the ligninolytic enzymes from
one fungus to another fungus which has a higher tolerance for
toxic soil, essentially making a super-bioremedial fungus.
If the enzymes were isolated efficiently, they could perhaps be
used by themselves in sites where it is too toxic for any organism
to prosper.
Then there is the untapped potential of the undiscovered fungi
around the world. As it is estimated that there are over
a million species, of which only 80,000 identified, the chances
are that there are fungi out there deep in rainforests which have
a far greater ligninolytic system and would be more useful to
the bioremediation research.
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